Good Morning, i am going to perform cell wall loosening enzyme assays in wheat anyone who has done and want to share his/her protocol will be highly appreciated. My focus is on XET and expansins. (xyloglucan-Endotransglycosylase)
While dealing with systematic classification we are following the standard taxonomic keys. A taxonomic key or guide gives us information about the identification, nomenclature, classification, and...
19 April 2024 9,054 0 View
I need to understand TGA graphs; can you help me with where to start?
31 January 2024 9,952 6 View
I performed an experiment involving the knockout (KO) of XYZ gene in CD34+ cells, followed by their differentiation into MKs. I am now seeking a reliable protocol for sorting the KO cells from the...
30 January 2024 8,319 0 View
I have about 40 draft genomes binned from metagenomes. I want to see their secondary metabolite production potentials. I used AntiSMASH in the command line. From the output files, I can see the...
29 January 2024 9,711 0 View
Is it possible to estimate the fluid flow behavior in the weld pool by analyzing the temperature distribution in the fusion zone?
27 December 2023 1,654 3 View
While performing CFA, I got negative and low factor loading for a few constructs in AMOS, but model fit indices are good. Is the result of the CFA model valid? Can i proceed with the study or do i...
19 December 2023 7,485 3 View
If you have used the software and are confident enough to add a comment here, 1. Can we use MAXQDA free as a trial version, like other 2. Is MAXDQA reliable for doing a qualitative analysis.
17 November 2023 7,349 2 View
For the acoustic design of an enclosure, is it enough if the natural frequencies of the enclosure do not match the frequency of the sound generated? Such an approach to vibration would be fine. Is...
30 October 2023 7,314 3 View
Procedure of making my samples. i Weighed 1 g of dextran with a molecular weight of 1000Da, add 4 mL of water, and dissolve in a 550C water bath. After dissolution of dextran properly i added...
11 October 2023 7,459 0 View
Within construal level theory literature, the construal level manipulation check is often takes place with scales such as behavioral identification form (BIF). This scale measure abstract vs....
05 October 2023 5,539 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View