Hallo Fawaz Al-Shaheri,

usually the use of your buffer system is depending on what system you use. pH and ionic strength have a large influence on the accuracy, sensitivity and precision of your microarray. Can you please give some further details, what kind of array you are planning to do? Especially the immobilization technique and the read-out technique are much depending on the buffer system and there is no buffer that works for all microarrays. What will be the signal generation step? Is it fluorescence or enzymatic detection? Sometimes you can also add agents to prevent non-specific binding. Usually these parameters need to be optimized, we did this with DoE some time ago:

Article Optimization and Evaluation of a Hapten Microarray Using Che...

For a first test, a PBS buffer is a good choice for starting as an incubation buffer. as washing buffer PBS with Tween 20 (0.05%) it should do.

Be aware that you have to block the microarray after printing, depending, which immobilization technique you use!

Our PBS buffer was usually

Na2HPO4 10 mM

NaH2PO4 70 mM

NaCl145 mM

pH = 7.60

Prepare the buffer freshly and don't use them longer then a week.

Hope this helps!

Best regards

Peter