How we can know that the column used in HPLC has a good efficiency through the shape of peaks in chromatogram?

11 November 2018 0 9K Report

I need to know if my column which i used in the analysis with high efficiency or no.

Badges
Science method

More Ahmed M. Al-Anbakey's questions See All

Can anyone provide me geometry of an axial pump with induced with mesh and setup using CFD fluent?

I am working to simulate axial pump with inducer using CFD fluent and I need help to known how can create axial pump that mean how can rotate impeller with fixed the pipe wall under transient...

01 February 2020 9,005 3 View

Does anyone teach a course on desertification?

Dear researchers I am going to teach a course on desertification but i need a help and somebody to share with me his/her experiences on teaching such a course. I would like to learn some teaching...

31 December 2019 2,588 4 View

I want to simulate journal bearing using Fluent CFD?

I am working on concentrated to model journal bearing therefore I need to simulate it using Fluent CFD. I need to help me to create, mesh and setup this journal bearing please how I can to do this...

11 December 2019 7,741 2 View

A request for an online course in principles of drug-protein and protein-protein molecular dynamic simulations?

Dear All, I am looking for an online course or website teaching the principles of molecular dynamic simulations. In specific, generating protein-protein and/or small drug-protein docking models...

10 November 2019 8,437 3 View

Is there a quantitative measurement of pH in cell culture using Phenol Red spectrophotometry?

Hello! I'm trying to determine the pH of a cell culture plate using phenol red present in the medium (MEM) and a plate reader. However, phenol red's absorbance changes depending on the pH. Is...

05 June 2019 7,675 2 View

Is there a website to obtain a Geological Map of Saudi Arabia (as shapefile)?

I am looking for a map in the resolution of Geological Formations, not according to the age of deposits in the format of shapefile or good resolution image. Thank you in advance.

01 February 2019 2,606 1 View

I want to know how nanomaterials can be used to get rid of oil contaminants?

I need accurate details for the purpose of conducting some technical tests that concern some of the problems facing the research

10 November 2018 1,486 4 View

What is the most suitable high thermal capacity materials for concrete?

I am looking for material with high thermal capacity, which is cheap and could be used in concrete and blocks?

10 November 2018 1,890 3 View

Two-Phase Flow?

Can any body tell me how to start simulation of water nucleation using COMSOL Multiphysics?

09 October 2018 6,084 5 View

Hi all, please I am quite confused regarding what is the difference between Effectiveness vs Functionality in Computer Science (Network Security)?

Well, after some researches I got that understanding: we can measure the Effectiveness of the security system through analyzing its works without facing the system to any threats or attacks. And...

07 August 2018 2,639 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View