We combined classical genomic DNA extraction methods and commercially available DNA affinity column, replaced the de-waxing by dimethylbenzene with water-bath, designed a fast and improved genomic DNA preparation method. We also extracted genomic DNA from paraffin embedded cervical cancer tissues, and checked the quality of DNA by agarose gel electrophoresis and polymerase chain reaction detection.

What is your sample/ tissue you are extracting the DNA? This would be a very important information.

We are using Manual method for DNA isolation i.e. Phenol-Chloroform method. It is a very good method & give us good quality and quantity of genomic DNA. If want we can send you protocol. 

Hi there 

can you please mention which method you are using. As CTAB method is one of the best methods that you can optimize according to your requirements. Also if you let us know about the sample that would also be helpful in answering.

The simplest and easiest way is that in the final step of DNA isolation, is to elute your DNA less volume of buffer/water e.g. in 50-80ul then automatically concentration will be high. Better quality could be achieved by using a better isolation kit and isolation in sterile conditions. Hope it helps.

Improving the quality of DNA mean to remove other contaminants from it.  Generally treating DNA with RNAses is recommended to yield high-quality DNA. Secondly, if you are using manual method like phenol: chloroform then pipette out the aqueous phase (DNA containing phase) very carefully so as no traces of organic phase (protein and lipid containing phase) can be transferred to the next step.

hihttps://www.researchgate.net/.../How_we_can_increase_yield_of_recover_DNA_and_...

for high quality and good DNA extraction, there are many commercial different kits can be used, for higher yield u can use manual DNA extraction protocol like salting out or phenol/chloroform.

To  get good quality DNA  from reminiscent sample , Chelex Resin is best for amplification of DNA.. The  method is eco friendly and don't require phenol chloroform or spin columns.

Jameela ,its all a question of type of material you want to extract from. CTAB is the best but can add modifications to it to get rid of polyphenols or carbs by including PVP/B-mercaptoethanol /increasing chloroform steps, then eliminating RNA with RNAses

All thanks  Fortunate Mufunda 

www.labome.com/method/DNA-Extraction-and-Purification.html

hello doctor i agree with Fourtunate 

We combined classical genomic DNA extraction methods and commercially available DNA affinity column, replaced the de-waxing by dimethylbenzene with water-bath, designed a fast and improved genomic DNA preparation method. We also extracted genomic DNA from paraffin embedded cervical cancer tissues, and checked the quality of DNA by agarose gel electrophoresis and polymerase chain reaction detection.

Improving the quality of DNA means to remove other contaminants from it.  Generally treating DNA with RNAses is recommended to yield high-quality DNA.