We are working on THLE-2 cell line in a project. We are using BEGM Bronchial Epithelial Cell Growth Medium BulletKit. The cells are in their thirteenth passages. Recently, cells dividing is slow down.
I want to add a source term as a body force to the momentum equation in Ansys CFX for modeling the VGs and I don't know how do it !!
21 February 2021 2,971 4 View
Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...
16 February 2021 1,594 3 View
In a typical moderation model with one moderator, is it a problem if the moderator and the dependent variable show a strong correlation (say 0.85)? What if a strong correlation exists between the...
27 January 2021 4,937 4 View
I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.
06 January 2021 5,769 3 View
Hi everyone, Does anyone know up to what reduction ratio of gear does a motor remain back-drivable? I want my robot to have safe interactions with the environment and other humans. Can you give a...
26 December 2020 7,142 3 View
Hi all; I've started using DigSilent these days. However, I'm wondering how can I export the results with high quality the same as MATLAB plots as I currently do. Thanks
19 December 2020 2,666 2 View
Hello! I am trying to define/assign initial stress to a coating I modeled on a substrate. I defined the coating using "skin" function. I have tried "predefine filed" in load section. But it only...
10 December 2020 2,921 8 View
Hello! I'm trying to model a multi-layer coating on a substrate in abaqus. The layers are homogenous and isotropic. My question is how should I approach the modeling? WHAT I'VE TRIED SO FAR: I...
09 December 2020 1,235 3 View
Calculate the Nusselt number using the COMSOL Program
09 December 2020 3,354 3 View
I am gonna use Li2S8 catholyte to introduce active material to cathode instead of using sulfur. May anybody kindly guide me about the way I can calculate the amount of sulfur (g/cm2) loaded on the...
07 December 2020 7,556 2 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View