We are working on THLE-2 cell line in a project. We are using BEGM Bronchial Epithelial Cell Growth Medium BulletKit. The cells are in their thirteenth passages. Recently, cells dividing is slow down.
Is a person with a history nodular gastritis, which has been through nodular regression (according to endoscopy) because of sucessful H.pylori treatment, has the same prognosis as a person with no...
20 May 2024 4,192 0 View
For a ship panel, I want to consider the initial deviation (thin-horse mode) to have a better analysis. How can I apply initial deviation from Rigo method in Abaqus?
19 May 2024 1,358 1 View
The question inquires about the potential use of the sol-gel technique for producing fiber preforms (rods) and whether its application is limited solely to volume optics. The sol-gel technique...
15 May 2024 9,211 0 View
One of the most important analytical tools often used by management executives for decision making is decision tree analysis. The most well-known approach to solve decision tree problems is the...
08 May 2024 6,619 4 View
I want to know how peak and rms current of diodes and capacitors are calculated in terms of output current? if you know good reference with details, please share here or tell me.
04 May 2024 6,277 1 View
The question is about the safety mechanisms in place within a car battery pack to manage and reduce pressure after a cell explosion. This is a critical aspect of battery technology, particularly...
18 April 2024 3,376 1 View
what are more desirable journals for Russian researchers in structural engineering field to submit their papers? thank you.
13 April 2024 5,004 2 View
Hi, What is this organism? I found it in Quaternary sediments!
25 March 2024 8,298 7 View
Guidance, navigation, and control systems are used in many vehicles to achieve precision, reliability, stability, mission path planning, and ... . what are the primary objectives of GNC systems...
24 March 2024 5,946 3 View
Hello, Which are some AI tools that researchers can use in writing scientific paper?
23 February 2024 1,792 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View