Mahdi Shekari

4 Questions 1 Answers 0 Followers

Questions related from Mahdi Shekari

How we can accelerate expansion of THLE-2 cell line?

We are working on THLE-2 cell line in a project. We are using BEGM Bronchial Epithelial Cell Growth Medium BulletKit. The cells are in their thirteenth passages. Recently, cells dividing is slow down.

10 February 2022 2,929 0 View

What are the steps of Sanger sequencing after double stranded plasmid vectors isolation (after cloning inserts in E.coli)?

Company supplied just forward primer for Sanger sequencing. I guess, there is no needs to amplification inserts using PCR thermocycler because it was done by bacteria itself. Now, should I begin...

24 May 2021 8,867 2 View

We would like to purchase OUMS-29 cell line- Hepatocytes (https://web.expasy.org/cellosaurus/CVCL_9130). Do you have any idea about the seller?

We have not found related company for purchasing the cell line.

09 October 2019 8,485 1 View

Do you think designing gRNA for targeting F8 promoter can be practical? Have you had any successful experience with targeting a gene promoter?

We plan a gene knockout experiment with CRISPR Cas9 technology.

02 October 2019 9,487 1 View