Hi

I try to cut DNA due to cloning to vector. I have to use SmaI and BgIll, they have 100 % activity at cutsmart buffer and NEBuffer, respectively. If I use only one buffer, either smal or BgIll have very low activity(10 %). So, first I cut DNA using SmaI and did prification to remove restrict enzyme and buffer. After purification, I cut DNA using BgIll equally. Then, I check DAN band by electrophoresis, I couldn't detect any band. I think DNA is washing away during purification. Is there any method when using different buffer activity restrict enzyme???

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