Lee Byunghoon

5 Questions 4 Answers 0 Followers

Questions related from Lee Byunghoon

Could anyone give an advice about confirming transformation?

Hello I have tried introduce pCL55-itet which is subcloned into B. subtilis ISW1214. pCL55-itet has ampicillin resistance marker So, I incubate B. subtilis ISW1214 on TSA + 100 mg/L ampicilin...

08 August 2018 5,946 3 View

Is DH5-a resistant to erythromyhcin??

Hello! I wanted to know if anyone has ever experienced anything like this. I have tried subcloning erythromycin resistance gene to pCL55-itet vector. I confirmed that subcloning was success. I...

07 July 2018 5,480 4 View

How to use restrict enzyme which have different buffer activity?

Hi I try to cut DNA due to cloning to vector. I have to use SmaI and BgIll, they have 100 % activity at cutsmart buffer and NEBuffer, respectively. If I use only one buffer, either smal or BgIll...

06 June 2018 2,963 14 View

Could anyone help me to choos bacilus sp. selective media?

Hello. I'm trying to isolate bacillus sp. form me-ju. I would like to get bacillus sp. by using selective media. I will appreciate if anyone let me know which media is best for!

12 December 2017 3,265 3 View

Can anyone help me to design a specific primer?

I try to design specific primer to distinghuish two speicies. However, their housekeeping gene is highly conserved. It's hard to find polymorphic site. Finally, I found different region. 1....

11 November 2017 8,137 3 View