Hey i am doing a project to find the most suitable housekeeper gene or gene combination for ischaemic rat kidney and toxic injury rat kidneys. I have 10 genes wich i am testing and 48 samples run in triplicates with standards (serial 10 dilutions) as well.

I am trying to use qbase+ for inter run calibration but it wont let me do it prior to normalisation. But the whole point of my prokjject is to find a suitable gene to normalise agaiinst. Hence i do not have referencen gene at present. I guess in other words, my question is about how to do inter run calibration when I am using a sample maximisation method.

Also have you seen this equatio N0 = Nq / Eamp^Cq. Is this a good way of calculating relative quantities to be used as input values in qbase+ and normfinder ?

Many thanks for your help :)