Can Dess-Martin Periodinane be used in DMF or THF?

After seeking for references, most of the solvents for Dess-Martin is DCM, but my compound has a poor solubility in DCM. Because my compound is acid-sensitive, I prefer not to use IBX to oxidize...

17 January 2021 3,586 3 View

The correct way to measure moss humidity in field?

I want to record the humidity of moss in the field within a few months, i am thinking I can use the Humidity recorder, placed on the moss surface, but is this the correct way to measure moss...

08 April 2019 5,491 1 View

Does any tardigrades can live in acid/base environments? Such as the soil of hot spring?

Hello, I want to try to find the water bears in the soil, but I am considering about the water bears that can survive in a hot and acid/base environment? Thanks!

11 January 2019 5,216 4 View

Tissue in liquid nitrogen can be used for pathological analysis?

I am concerned that hepatic tumor tissue stored in liquid nitrogen for 3-5days can be deflozen and used for pathological analysis to estimate the invasion condition

25 January 2018 3,130 2 View

Do anyone of you have a scale named cognitive flexibility scale?

19 February 2017 3,505 3 View

Any suggestion for buiding the orthotopic lung cancer?

07 August 2016 4,644 4 View

Can anyone explain reason for HPLC column back pressure and RT shift?

I recently observed the shift of the whole profile little by little due to the passage of time / long term use of the column. In 2 weeks, the shift became 0.4min much later and the back pressure...

15 March 2016 7,747 3 View

No title

22 February 2016 5,802 7 View

What's your perception of teachers' rights and your knowledge about this issue?

Can educators share your views on teachers' rights by giving your 1. personal thoughts and 2. theoretical knowledge in this area? TQ

09 January 2013 8,963 3 View

How many types of explanations are there for the spin angular momentum?

In the Quantum Mechanics, the origination and essence of the spin angular momentum remain a mystery. Recently some viewpoints are in the violent competition. The first viewpoint deems that the...

01 January 1970 5,793 40 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View