Can Dess-Martin Periodinane be used in DMF or THF?

After seeking for references, most of the solvents for Dess-Martin is DCM, but my compound has a poor solubility in DCM. Because my compound is acid-sensitive, I prefer not to use IBX to oxidize...

17 January 2021 3,586 3 View

The correct way to measure moss humidity in field?

I want to record the humidity of moss in the field within a few months, i am thinking I can use the Humidity recorder, placed on the moss surface, but is this the correct way to measure moss...

08 April 2019 5,491 1 View

Does any tardigrades can live in acid/base environments? Such as the soil of hot spring?

Hello, I want to try to find the water bears in the soil, but I am considering about the water bears that can survive in a hot and acid/base environment? Thanks!

11 January 2019 5,216 4 View

Tissue in liquid nitrogen can be used for pathological analysis?

I am concerned that hepatic tumor tissue stored in liquid nitrogen for 3-5days can be deflozen and used for pathological analysis to estimate the invasion condition

25 January 2018 3,130 2 View

Do anyone of you have a scale named cognitive flexibility scale?

19 February 2017 3,505 3 View

Any suggestion for buiding the orthotopic lung cancer?

07 August 2016 4,644 4 View

Can anyone explain reason for HPLC column back pressure and RT shift?

I recently observed the shift of the whole profile little by little due to the passage of time / long term use of the column. In 2 weeks, the shift became 0.4min much later and the back pressure...

15 March 2016 7,747 3 View

How to use lipofectamine3000(Invitrogen) to perform the packaging of lentivirus?

29 February 2016 7,916 1 View

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22 February 2016 5,802 7 View

What's your perception of teachers' rights and your knowledge about this issue?

Can educators share your views on teachers' rights by giving your 1. personal thoughts and 2. theoretical knowledge in this area? TQ

09 January 2013 8,963 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View