Why choose Phosphoric Acid/ Hydrochloric acid Acid/Sulfuric acid as the acidify agent in the extraction of short chain fatty acid?

18 July 2023 0 2K Report

The theory basis of this processing procedure seems unclear to me, and I have much difficulty in exploring the reason……

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How to determine the knots when fitting the Expo-rational B-splines (ERBS) curve?

Exporational B-splines, a new type of spline function with better mathematical properties in theory (refer to [1]), require consideration during curve fitting due to the crucial impact of knots...

15 April 2024 2,129 0 View

I have a question about concatenating sequence runs in MEGA X. Can you assist me with this?

I concatenated four sequences. Can I find the best model for the concatenated sequences and then run the maximum likelihood tree with the best model in MEGA X?

14 December 2023 7,498 0 View

MTT assay treat with protein sample, but it always precipitate which makes low cell viability?

As title, my sample is kind of plant-based protein(pI=4.5) and its hydrolysates and glycated protein. I treat my sample with CACO-2 cells in MTT test, but I found that my sample is ph-sensitive,...

13 May 2023 4,592 2 View

How to modify the Amber code to save the trajectory of solute only?

Dear Amber user, I want to study the interactions between protein and ions. I am running a simulation with protein, ions and water and I want to save the trajectories of protein and ions. I know...

26 March 2023 5,804 2 View

How to make Zn move along with Zn-binding domain?

Dear all Gromacs user, I am using Gromacs to run NVT and NVT equilibrium. Before the equilibrium, my input gro file shows all atoms (proteins, Zn, Na and Cl ions, and waters) stayed in the box...

17 February 2023 4,753 1 View

Can anyone suggest a good method for human lung macrophage isolation?

I am planning to isolate macrophages from human control and ARDS lungs. Can anyone suggest a good method? Thanks!

08 February 2023 4,200 0 View

How to add a zinc ion to a protein pdb file?

Dear all, I am running MD simulation of a protein containing a zinc finger (ZnF) domain. I got its all-atom pdb file and I know there are 4 sulfur atoms in 4 cycteins of ZnF domain interacting...

10 January 2023 8,701 4 View

How to run molecular dynamic simulation in 100 nsec?

Dear All, Could you tell me how to run MD simulation by any software. I have DS, VMD in hand. But I try to run 30 nsec in DS that spent 6 days, is that right? The reviewer asked me to perform...

30 October 2022 2,089 5 View

Ammonium Bicarbonate Hydrogenation to Formate in a PFR reactor converging but with no reaction happening on Aspen Plus Simulation?

Hi, I am currently conducting a simulation of CO2 conversion into Formic acid via hydrogenation of ammonia-based system. The reactants includes the aqueous ammonia and flue gas of CO2. There are...

08 October 2022 4,919 1 View

How to elution the His-tag protein ?

I tried different imidazole to elution the his-tag protein from Ni-NTA but failed. Does anyone encounted this problem before and find a solution ?

20 September 2022 5,392 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View