6 Questions 4 Answers 0 Followers
Questions related from Qiaoyou Weng
I tried different imidazole to elution the his-tag protein from Ni-NTA but failed. Does anyone encounted this problem before and find a solution ?
21 September 2022 5,374 3 View
I am concerned that hepatic tumor tissue stored in liquid nitrogen for 3-5days can be deflozen and used for pathological analysis to estimate the invasion condition
26 January 2018 3,221 2 View
Does anyone has the problem with bioluminescence? The A549 cells were transduced with Firefly luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles and inject into the nude rats lung,...
16 September 2016 7,342 2 View
I tried to build this model by percutaneous injection to the right lung under the X-ray. But cancer cells are spread to the pleural cavity and left lung. Has anyone has any good idea?
08 August 2016 4,730 4 View
"Plasmids were co-transfected with lentiviral packaging vectors into 293T cells using Lipofectamine 3000 according to the manufacturer’s instructions", but there is no details about how to use the...
01 March 2016 7,983 1 View
When I pick a single from a freshly streaked selective plate and incubate a starter culture of 5mL LB medium containing the appropriate selective antibiotic for 8H at 37 ℃ with vigorous...
23 February 2016 5,875 7 View