Hi,
I am trying to use to fluorochrome impregnated beads as internal counting beads during flow cytometer cell counts. So, I know how many beads I add to the tube based on the information provided commercially, but I do not get the exact bead count or counts not even close, under microscope the beads appear to be clumped together, so flow cytometer might consider as single events and that could be the reason I am not getting absolute numbers, so I vortexed the beads before counting still it did not improve the cell count.
Kindly help
Thank you
Vinitha
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