Adoption of Innovation Academical Article?

Dear researchers, I am preparing a thesis and one of my paragraph in my academic research could be about "adoption of innovation" I did not found any academic article listing in FNEGE 2019 Could...

26 April 2020 7,995 5 View

Sample size, size effect and statiscal power- U mann Whitney test?

Hello. I study Psychology in the University of Paris-VIII(Master 2 in developmental Psychology). I work on the school engagement of different groups. I have some questions about how to interprate...

04 June 2019 1,146 1 View

Who is using RapID system for mouse identification?

16 August 2017 9,068 1 View

Does it exist an application for in vivo follow up?

08 August 2017 6,932 2 View

Can IP injection of mouse cancer cells induce inflammation in mice ?

02 November 2016 4,368 3 View

Do we need Matrigel for SW48 colon cancer cells SC-injection?

30 August 2016 1,887 3 View

Why NOD-Scid do not tolerate irradiation while nude mice do ?

14 June 2016 9,791 5 View

In order to study changes in the proteome of biological fluids, what is hte best methods, 2D-DIGE or liquid chromatography?

In order to study changes in the proteome of biological fluids, what is hte best methods, 2D-DIGE or liquid chromatography?

10 May 2016 9,492 1 View

How do I perform in vitro irradiation on GBM cells?

15 April 2015 3,525 3 View

How to identify a protein X co-immunoprecipitated with a protein of first interest?

20 February 2015 8,921 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View