How to upload CDS and Protein sequence together in NCBI?
You can use u tube
I have collected intraspecies interaction between adult, subadult and juvenile of vulture during their feeding. But I have only noted the frequency of interactions not their types. I am confused...
29 June 2022 8,556 2 View
I have tried making a 5% solution (by weight) of PVA with DI water. Below are the steps that I followed: (a) Prepared a 5% solution of PVA with DI water (b) Magnetic stirring done for 6 hours at...
08 February 2022 534 4 View
Hello all, I have come across in literature of CFD modeling of residual ( capillary ) trapping phenomenon in carbon capture and storage that surface tension force and contact angle is considered...
13 December 2021 9,923 0 View
Basically I am here for few months. I am trying to collaborate other professor but I am always failed. So can you share me how can I find international professors to collaborate in my...
07 July 2021 1,091 6 View
Hello everyone, I have a very naive question. I am working with inhouse code and adapting it for combustion studies ( with flow dynamics ). However, I am unable to find any simple test case to...
24 June 2021 2,571 4 View
Hello. I am currently running haemolysis assays to measure inhibitory effect of some complement regulatory proteins. The assay involves complement activation by either the classical pathway (CP)...
02 May 2021 8,245 0 View
Hi, could anyone let me know what's the total number of bioactive secondary metabolites that have been discovered from microbes by now? Also please provide me the link, if you have it. Thanks!
17 April 2021 1,957 6 View
Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...
25 February 2021 8,513 5 View
Please see the attached picture that show how some Ranitidine Hydrochloride tablets have gone horribly wrong within 12 months of real time stability studies. The leak test still complies, but,...
07 October 2020 4,433 14 View
Hello all, I am looking for an method / algorithm/ or logic which can help to figure out numerically whether the function is differentiable at a given point. To give a more clear perspective,...
11 September 2020 6,235 14 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...
04 July 2024 9,875 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....
30 June 2024 8,650 0 View
Hi, everyone I would like to know if there is there any bioinformatic tool that can download batch DNA seq using imported NCBI accession numbers (upto 100 sequences) and translate them into...
19 June 2024 1,917 2 View
I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...
12 June 2024 8,822 1 View
Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...
03 June 2024 9,451 3 View
when I insert my primers in primer blast site and push the button "get primers" I just reach different errors and I don't know what the problem is!
22 April 2024 8,001 5 View
We have assembled a chloroplast genome of angiosperm and submitted the annotated assembly to NCBI GenBank. Within two-working days we got accession number for that submission. GenBank in their...
21 April 2024 9,974 0 View
Which software to compare two genomes
09 April 2024 4,571 2 View
