Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Numerically investigating whether a function is differentiable?

Hello all, I am looking for an method / algorithm/ or logic which can help to figure out numerically whether the function is differentiable at a given point. To give a more clear perspective,...

11 September 2020 6,072 14 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Cloning clusters in streptomyces?

Is someone working with cloning big size biosynthetic gene cluster (>50kbp).Quite difficult to fish the whole cluster??? Any tricks so far?

23 July 2018 5,993 16 View

What are the possible ways for utilizing municipal waste in agriculture?

other than composting.

26 June 2018 6,682 18 View

What is the minimum sample size required to include four dummies in multiple regression?

We have collected population growth data of a carnivore from five provinces and would like build a multiple linear regression model (OLS) with two explanatory variables. We have 7 observations...

07 January 2018 4,118 5 View

Difference between phase-field and level set method?

May someone help in understanding the difference between phase-field method and level-set method? Thank You

03 January 2018 9,451 5 View

Can planted forests provide multiple ecosystem services? and to what extent?

13 November 2017 5,708 15 View

Spinoidal decomposition using phase field modelling?

Hello all, I am trying to simulate spinoidal decomposition using phase-field method. Most of the literature I could have given equations in dimensionless form. However, I intend to solve them in...

05 November 2017 507 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Once I annotate a genome with PROKKA, where do I uplaod it?

I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...

29 December 2020 3,976 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

How can I design primers of some micro RNAs online ?

How to use NCBI to design primers for specific Micro RNAs for quantitative analysis and how to check the efficacy of the selected primer before working

24 December 2020 180 3 View

How can I know which sequence is the correct one if there are lot of predicted sequence in database for my target gene?

Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...

23 December 2020 5,531 12 View

Which is the best mitochondrial genome annotation tool? Can anyone provide the list?

I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.

19 November 2020 8,849 1 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Why will only one of three genes amplify CRISPR-Cas9 triple knock out DNA preps?

Working to genotype CRISPR-Cas9 triple mutants. One of the three genes amplifies very well, the other two won't. I have tried three different primer pairs for each of the two genes that won't...

12 October 2020 2,429 3 View

Any suggestions of bioinformatic tools to identify/predict the effect of mutations observed on the function of proteins?

04 October 2020 1,552 4 View

Donwloading from NCBI?

Dear everybody, I'm posting this question because I'm facing a problem with the NCBI database. I should download all the species that belong to a particular phylum (Nematoda) with name and NCBI ID...

30 September 2020 7,808 3 View