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Hi guys, I am trying to culture mouse embryonic stem cells ( JM8A3; C57BL/6N ) on coverslips coated with 0.1% gelatin for confocal microscopy. The cells grow well but once I start washing the...
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Hello everyone, I have unexplained phenomenon in my data regarding one cytokine. The level of this cytokine by Qpcr is very high in knockout more than WT mice in naive condition and after...
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Hello guys , I am doing EMSA , and I need to design probes , I have the oligo sequence that is expected to be the binding site for the transcription factor. However , I don't know what is the...
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Hello guys, I am running western for the 3rd time for the same protein. First two times I can see clear one band. It is inducible protein. However, on the 3rd time, I have two bands , instead...
01 February 2018 6,004 2 View
Dear fellows, I am facing a problem using Isotype control, for example, when I run flow , the signal in un-stimulated samples is 10%, and in stimulated samples is 12%. However, the isotype...
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Hello, I am infecting mice orally with salmonella 1344. I used two doses 10e4 and 10e6. I did fecal CFU on day 3 and day 5 post infection to detect salmonella titre in feces. However, no colonies...
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Does it affect the efficacy of qPCR data if I prepared syber green with primers and kept it for 1-2 hours before adding cDNA and running QPCR? Thanks
03 November 2015 3,444 8 View
