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Hi guys, I am trying to culture mouse embryonic stem cells ( JM8A3; C57BL/6N ) on coverslips coated with 0.1% gelatin for confocal microscopy. The cells grow well but once I start washing the...
07 January 2020 7,227 4 View
Hello everyone, I have unexplained phenomenon in my data regarding one cytokine. The level of this cytokine by Qpcr is very high in knockout more than WT mice in naive condition and after...
19 September 2018 5,764 3 View
Hello guys , I am doing EMSA , and I need to design probes , I have the oligo sequence that is expected to be the binding site for the transcription factor. However , I don't know what is the...
06 April 2018 717 10 View
Hello guys, I am running western for the 3rd time for the same protein. First two times I can see clear one band. It is inducible protein. However, on the 3rd time, I have two bands , instead...
01 February 2018 5,997 2 View
Dear fellows, I am facing a problem using Isotype control, for example, when I run flow , the signal in un-stimulated samples is 10%, and in stimulated samples is 12%. However, the isotype...
23 November 2017 7,799 1 View
Hello, I am infecting mice orally with salmonella 1344. I used two doses 10e4 and 10e6. I did fecal CFU on day 3 and day 5 post infection to detect salmonella titre in feces. However, no colonies...
04 March 2016 3,830 3 View
Does it affect the efficacy of qPCR data if I prepared syber green with primers and kept it for 1-2 hours before adding cDNA and running QPCR? Thanks
03 November 2015 3,433 8 View
