How to transversally cut mice carotid artery?

19 May 2017 1 1K Report

I've tried to perform both wire injury and carotid artery ligation models of hyperplasia. However i was not able to get transverse section of the carotid artery for histology analysis. Any tips? thanks in advance

Mamdouh Afify

Dear Ignacio,

I recommend you to use a piece of filter paper around the artery ( like susage) and you have to fixe it for 24 hr in formalin. It woud be easy for you to cut it in a transverse manner. Please embedd the specimen in paraffin personally.

best wishes

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