Does someone know which statistical test I should use to compare these groups having a categorical dependent variable?

I have two groups (10 participants in one and 12 in the other), each one viewed 1 stimulus (web page that present 4 products to choose between). One of the web pages has an orange label under one...

31 March 2020 2,812 4 View

How can I get started with PIC code simulation?

I've already asked a question like that and followed your advice. I started studying: Plasma Physics via Computer Simulation (C K Birdsall and A B Langdon) but I really think that I need again...

21 October 2019 6,835 3 View

How do I test my (stimuli material) independent variable against the dependent variable on SPSS?

Hi all, I am currently writing my dissertation and I am struggling to analyse my survey in SPSS. I hope you can help me. I have created one survey, with 3 different stimuli material....

02 March 2019 9,851 1 View

Where can I find references about real topological vector spaces such that all vector subspaces are closed?

My interest is in characterization of spaces described in the question. Clearly the interesting case is about infinite dimensional real vector spaces.

31 January 2019 1,498 8 View

How do I remove debris from a beads suspension?

I have a suspension of 40 um beads (2 mL at 400 beads/uL) and I noticed that there are a lot of debris in it. I need to remove them as much as possible to obtain a suspension with no or few...

23 October 2016 7,569 3 View

Does anyone know a protocol for DNA extraction from saliva, hair and blood from dog?

Hello everyone, we are looking for a protocol to build up for the extraction of DNA from saliva, hair and blood samples collected from dogs, since extraction kits are not suitable for canine...

29 September 2016 4,382 6 View

Adhesion problem with beas-2b?

Hi everyone, I have adhesion problems to flask when I thaw the Beas-2b cells, someone has any advice for me? someone would be willing to give me a aliquot? Thank you Tommaso

05 April 2016 2,990 2 View

Does anyone know an updated gene ID converter?

Good morning to everybody, I am working on gene expression with a pattern of about 20,000 genes from Bos taurus that are encoded with a RefSeq mRNA ID. In order to use a program for the impact of...

03 September 2015 267 9 View

How does the length of the shortest path *in T* from node h to node k (if any) grow with their 'numeric distance' (k - h)?

15 September 2014 9,745 3 View

Problems with HUVEC

I'm working with HUVEC isolated from normal donors but I have some problems. I found a lot of variability in their responsiveness to my stimuli (e.g. TNF-alpha), is it possible to speak about...

21 March 2012 4,063 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View