How to titrate dengue virus?

25 March 2022 1 1K Report

Hello everyone,

I'm trying to titrate different serotypes of dengue virus. I'm currently using VERO CCL81 cells in plaque assay and TCID50 assay, but only dengue serotype 2 virus showed good results. I'd like to know another affordable and simple assay to titrate dengue viruses.

Muazzam Sheriff Maqbul

https://link.springer.com/protocol/10.1007/978-1-4939-0348-1_2#:~:text=Mosquito%20inoculation%20is%20a%20highly,an%20insectary%20to%20rear%20mosquitoes.

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Science method

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