How to prevent Band/Edge Pass filter emission on Pulsed Laser excitation?

We are observing emission from both the Edge and the Bandpass filters on excitation with a Pulsed Laser. The emission is significantly high with multiple/uncontrolled peaks superimposed on the...

01 December 2020 5,449 1 View

Picking a resist to act as adhesive bw SU8 and silicon?

Hi, I have a silicon die with 6-10 um high SU8 features (cured) and I want to bond that die to a plain silicon die (or glass, I am actually not particular about the top die). If I am bonding to...

15 October 2020 3,118 1 View

How to take the layer of glass in solar cell?

I'm willing to re-create a perovskite based solar cell but it is having glass in it and I don't know how to take glass in silvaco atlas.

06 May 2020 6,238 12 View

Is log i vs log v (scan rate) an appropriate parameter for defining the capacitive and battery behaviour.?

According to the power law i=av^b, for a material (reduced graphene oxide) to be capacitive the value of b=1 whereas b=0.5 for battery like behaviour. However, the CV shows a quasi rectangular...

26 February 2020 4,592 4 View

Hi I am using Hofstede VSM 94 model and bit confused in calculating the different dimensions among regions??

Please provide me with a example to compute it.

16 September 2019 3,423 2 View

In which solvent CdS-TiO2 composite may dissolve?

want to perform CV measurement.

14 August 2018 5,773 2 View

How to filter reads in fastq for small RNA?

Hi I am analyzing a small RNA dataset. I read in a paper that reads occurring less than 5 times were filtered. I was wondering how to it exactly. If anyone can help Thanks in advance

07 August 2018 835 2 View

What is the different between LNCaP and LNCap-LN-3 Cell Line? Is LNCaP-LN3 cell line can be use to develop prostate cancer model in nude mice?

In the literature, LNCap is lymph node metastasis cell line obtained from Caucasian male and LNCaP-LN-3 is metastatic subline of LNCaP cells derived by orthotopic implantation. I want to know...

04 July 2018 2,779 3 View

Arabidopsis leaves are not bleaching in high salt concentration?

Hi there, I am doing Arabidopsis leaf disc senescence assay by using 300mM and 500mM NaCl solution. After punching leaf discs, I am keeping them in distill water (Control), and in different salt...

07 June 2018 9,061 3 View

How to do ChIP qPCR data analysis to study Histone modification?

06 February 2018 6,839 2 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View

What may be the best title and reference materials for writing an article on "Streaming, pupil subcultures and adaptations"?

I am writing an article on Streaming, pupil subcultures, and adaptations.

24 July 2020 7,316 2 View

What would be the exact sources of literature review for the field of class subculture and educational attainment?

What are the literature review sources of class subculture and educational attainment? Which on social science theory is linked with this subject matter? and how?

24 July 2020 4,812 2 View

What statistical analysis would I need for a questionnaire?

Let me first start by saying I'm dyslexic, two universities were unable to teach me research methods and statistics in a way that worked with my dyslexic limitations, and I've continuingly failed...

08 July 2020 8,712 6 View

Why i do get black particles in cell culture and i also have dead cells adhered to my grown live cells and its like just waves?

I grow LC-540 cells and my cells are clear when fully grown image 1, but when I trypsinize the same plate I see black particles image 2. When I subculture them on a new plate I don't see those...

02 March 2020 6,467 1 View

Why i do get black particles in cell culture and i also have dead cells adhered to my grown live cells and its like just waves?

I grow LC-540 cells and my cells are clear when fully grown image 1, but when I trypsinize the same plate I see black particles image 2. When I subculture them on a new plate I don't see those...

01 March 2020 2,018 4 View

Conditioning bone marrow mesenchymal stem cells?

Hi! I want to make conditioned media from BMSCs in order to study their paracrine activity in other type of cells. I made the CM when BMSCs were at 90% confluence. Simply, I aspirated basal media...

01 February 2020 1,211 3 View