Can anyone please suggest how can I procure 5beta-pregnane-3,20-dione standard? I have been searching different companies, mostly I have found the 5alpha form of the same chemical. I am currently intended to perform enzyme assay using progesterone 5beta reductase whose product should be 5beta-pregnane-3,20-dione. Kindly suggest me which technique I can use to justify the product formed if use the 5alpha-pregnane-3,20-dione standard which is a stereoisomer of my desired product.
You may contact Natural Remedies Pvt. Ltd., Bengaluru. It is not available in Sigma-Aldrich.
A Google search shows many options for me, including MilliporeSigma (as vendor, they supply it from someone else). See links below based on
CAS# 2483832-10-4 (also existing as 128-23-4):
https://www.sigmaaldrich.com/US/en/product/aablocksinc/aabh303716f6?context=bbe
https://www.cdnisotopes.com/5beta-pregnan-3-20-dione-2-2-4-4-17alpha-21-21-21-d8-9330?___store=default
https://www.chembk.com/en/chem/2483832-10-4
https://theclinivex.com/5b-pregnan-3-20-dione-2-2-4-4-17a-21-21-21-d8.html
https://isotope.com/steroids-and-hormones/5-beta-pregnane-3-20-dione-2-2-4-4-17-alpha-21-21-21-d8-dlm-9901-pk
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