How to prevent Band/Edge Pass filter emission on Pulsed Laser excitation?

We are observing emission from both the Edge and the Bandpass filters on excitation with a Pulsed Laser. The emission is significantly high with multiple/uncontrolled peaks superimposed on the...

01 December 2020 5,449 1 View

Picking a resist to act as adhesive bw SU8 and silicon?

Hi, I have a silicon die with 6-10 um high SU8 features (cured) and I want to bond that die to a plain silicon die (or glass, I am actually not particular about the top die). If I am bonding to...

15 October 2020 3,118 1 View

How to take the layer of glass in solar cell?

I'm willing to re-create a perovskite based solar cell but it is having glass in it and I don't know how to take glass in silvaco atlas.

06 May 2020 6,238 12 View

Is log i vs log v (scan rate) an appropriate parameter for defining the capacitive and battery behaviour.?

According to the power law i=av^b, for a material (reduced graphene oxide) to be capacitive the value of b=1 whereas b=0.5 for battery like behaviour. However, the CV shows a quasi rectangular...

26 February 2020 4,592 4 View

Hi I am using Hofstede VSM 94 model and bit confused in calculating the different dimensions among regions??

Please provide me with a example to compute it.

16 September 2019 3,423 2 View

In which solvent CdS-TiO2 composite may dissolve?

want to perform CV measurement.

14 August 2018 5,773 2 View

How to filter reads in fastq for small RNA?

Hi I am analyzing a small RNA dataset. I read in a paper that reads occurring less than 5 times were filtered. I was wondering how to it exactly. If anyone can help Thanks in advance

07 August 2018 835 2 View

What is the different between LNCaP and LNCap-LN-3 Cell Line? Is LNCaP-LN3 cell line can be use to develop prostate cancer model in nude mice?

In the literature, LNCap is lymph node metastasis cell line obtained from Caucasian male and LNCaP-LN-3 is metastatic subline of LNCaP cells derived by orthotopic implantation. I want to know...

04 July 2018 2,779 3 View

Arabidopsis leaves are not bleaching in high salt concentration?

Hi there, I am doing Arabidopsis leaf disc senescence assay by using 300mM and 500mM NaCl solution. After punching leaf discs, I am keeping them in distill water (Control), and in different salt...

07 June 2018 9,061 3 View

How to do ChIP qPCR data analysis to study Histone modification?

06 February 2018 6,839 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View