I'm working on silymarin gold nanoparticles and I am unable to understand if silymarin is conjugated with gold or not because it is showing no peak shift in UV and the pallet I obtain after drying is mostly gold and in very small amount.
Sorry, I am not a chemist. The chemistry part was done by a colleague of ours. Well, you will have to do absorption studies to make sure of the binding. You can confirm the binding by FTIR and spectroscopy. The following protocol is for garlic but is applicable to silymarin (New J. Chem., 2015, 39, 5003): "In a volumetric flask 250 mL deionized water was taken and 85 mg HAuCl4 (1 mM) was added which was followed by addition of freshly prepared aqueous garlic extract and 0.1mL (10 mM) NaBH4 solution under constant stirring. A gradual change in the color of the solution from yellow to pink was observed which indicated the formation of G-AuNPs. The reaction mixture was stirred for 4 hours to reduce gold completely. The reaction was optimized at 2 : 1 (v/v) gold solution to garlic extract." Regards
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