How to sterilize collagen solution?

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• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Polymer wear calculation?

What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?

04 August 2024 1,078 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

Why is it not common to see similar pairs in polymer sliding pairs?

If the pairs are similar, will it reduce the adhesion due to electric charges?

29 July 2024 3,185 2 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

Wrong out put for gmx x2top?

Dear users, I would like to simulate a zeolite structure in gromacs. I got the .cif file from IZA expanded in one direction in Materials Studio and exported a .pdb file. However, when I want to...

24 July 2024 367 4 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

Nicotiana benthamiana seeds sterilization methods for tissue culturing?

I have been trying to grow Nicotiana benthamiana via tissue culture. I used ethanol and sodium hypochlorite with different concentrations and time duration for seeds sterilization and lastly...

18 June 2024 1,756 3 View

Plant extract sterilization???

Dear researchers, I've been working on aqueous plant extract sample, then I autoclaved them in 10 min to sterilize and added some preservatives. However, some of them showed bacterial and fungal...

11 June 2024 1,040 2 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

Does autoclaving reduce the concentration of heavy metals in sewage wastewater?

I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...

10 June 2024 2,579 4 View

I noticed some white growth in the phosphate buffer that was prepared just a week ago. What could be the cause of this, and how can it be resolved?

The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?

10 June 2024 3,970 2 View

Do I need to autoclave 2X CTAB and Wash buffer?

I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...

09 June 2024 8,470 2 View

Could you please help me identify what might be wrong with my plaque assay?

I used Vero E6 cells and Mayaro virus. The virus was diluted in DMEM without FBS. The plaque media was 2X MEM, prepared with sodium bicarbonate and L-glutamine, adjusted to a pH of 4 with sodium...

03 June 2024 519 0 View