Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to efficiently precipitate platinum(IV) prodrugs from DMSO?

I am working with platinum(IV) prodrugs. Most of the synthetic procedures states that after most of DMSO is removed the residue should be treated with methanol and ether, giving the desired...

12 July 2020 1,577 3 View

What scale would you recommend to measure patient satisfaction with using innovative service?

I'm looking for a validated measurement tool, which could be used to assess clients' satisfaction with using an innovative service. Targeted user group - patients with mental health disorders.

04 June 2019 3,442 4 View

Is there a way to determine stability constant of an element - sorbent system (Resin) trough distribution coefficients or a gamma spectroscopy?

Hello, for already determined distribution coefficients in different systems aqueous solution/resin, via gamma spectroscopy, Is there a way to determine a stability constant in order to...

27 February 2018 1,414 3 View

What kinds of lateral grain distributions exist?

We're now preparing an article about MRI contrasting by magnetitenanoparticles and I'm puzzled, what kind of grain statistic can be usedto describe non-uniformity of lateral particle distribution....

10 September 2016 2,081 1 View

Where can I get this work: Dirichlet P.G.L. Abh. der Koniglich. Preus. Acad. der Wiss. , 1850?

14 April 2016 8,026 3 View

Can I seed H295R cells one cell in one well in a 96-well plate?

I am afraid that 1 cell alone would survive very difficult. Any recommendations?Should I use a medium rich in growth factors? To use a mix of old and new mediums? eg 50% of old medium and 50% of...

25 March 2015 792 3 View

What is the nonlinear refractive index of polycarbonate?

When calculating the critical power of self-focusing is requaired nonlinear refractive index, but my search didn't lead to success. Is there any information about it value?

03 December 2014 7,946 6 View

Does anyone know how to reliably differentiate between adrenal adenoma from metastatic RCC in adrenal gland?

IHC, EM or simple histology - everything is OK, but I need 100% of assurance.

11 October 2014 4,637 3 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View