In tissue culture, it is recommended that most hormones be added to the medium by filter sterilization. However, when a pH value of 5.8 was added to a stock solution whose pH was not set to 5 and around, the pH value was changed by 1 point, sometimes more. I tried to adjust it with HCL and NAOH precisely and carefully but I could not succeed.
Please look at the following below links which may help you in your analysis:
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7109460&blobtype=pdf
https://mynotebook.labarchives.com/share/BCHM2072%20Master/NjguOXwxMjgxOS81My9UcmVlTm9kZS8zOTI2NzMwMjc0fDE3NC45
Thanks
When you want to add growth regulators from Stock Solution to Media, the pH is adjusted with NaOH and HCl at a concentration of 0.1 normality for each of them. Thank you and good luck
How could you not succeed adjusting the pH with HCl and NaOH? The only way that can't work is if your solutions aren't what you think they are. What specifically did you do with NaOH and HCl, and what happened?
you can add the PGRs from the stock solution and then after that, you can adjust the pH by HCl and NaOH and then do autoclaving, hope it will works,
good luck
If the hormone used is not heat sensitive, the best way is to add it to the culture medium, then adjust the pH and finally autoclave. If the hormone is heat sensitive, you can test the rate of change and adjust the pH accordingly before autoclaving.
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