How to set an effective dose for any experiment related to toxicology in context of IC50, LC50?

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

What publications should I target as a psychology masters student in the UK?

I am writing a paper as a part of my course. I am new in London and was wondering that what publications should look upto?

21 July 2024 3,538 1 View

How effective has the United Nations been in addressing the conflict and its consequences?

After World War, we have seen the formation of the United Nations. The sovereign body for peacekeeping but with regards to the Russia-Ukraine war it is visible that the UN seems to be ineffective...

18 July 2024 4,674 4 View

What are the main obstacles to achieving a ceasefire or peace agreement between Russia and Ukraine?

It has been a quite long time since the Russia and Ukraine war has been going on and if we see the context of Geopolitics and International Relations we don't see a mediation between the two...

18 July 2024 6,737 4 View

How to decide whether the refinement is correct or not, based on Rwp and Rexp factors by Fullprof?

One of the papers I read by Toby, where (title of the paper was "R factors in Rietveld analysis: How good is good enough?"), he tells us that to get good chi square value, you must have low Rwp,...

17 July 2024 9,668 4 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why do I see products of the wrong size?

I am encountering an issue with my PCR band size. The expected band size is around 450 bp, but I am consistently obtaining bands near 2,000 bp. There are no bands in the negative control, so...

30 June 2024 295 0 View

How to pellet adipocyte spheroids?

I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....

27 May 2024 1,303 1 View

If Concentration of DNA is 85ng/ul then calculate its concentration for 50ul reaction using Phusion Flash Master Mix?

can any budy explain how i calculate the DNA concentration for the 50ul reaction?

22 May 2024 9,557 1 View

Can I find a fast journal (indexed in scopus and without fees)?

Good greeting. I want to publish a paper review in a journal specialized in food, health, toxicology, or even environmental sciences, provided that it is indexed in Scopus. I hope you can help...

19 May 2024 4,426 4 View

Best method for extracting RNA from low biomass of Ectocarpus (filamentous brown algae)?

Hello everyone! I am currently working with the model organism Ectocarpus (filamentous brown algae). Our team is trying to standardize a protocol for RNA extraction using low biomass (around 10...

18 May 2024 3,862 1 View

Hi I want to calculate the osmolality of my buffer, can anyone help me with this ?

My buffer components consists of 150mM Nacl 5mM HEPES 20mM EDTA and volume is made up to 10ml by ultrapure water what is the osmolarity of this soln ?

16 May 2024 5,245 0 View

Problem for methylation detection using qPCR?

Despite satisfactory qPCR curves for the positive control and calibrator, I observe a lack of signal from the VIC dye (reference gene) in my experimental samples after bisulfite conversion. I have...

13 May 2024 8,026 2 View

How to extract RNA and amplify cDNA from MS2 bacteriophage (ATCC 15597-B1)?

I am trying to extract RNA from bacteriophage MS2 filtrate of 10 log pfu/ml (propagated in E. coli C-3000 host) using Trizol and Qiamp viral RNA extraction kit. The RNA concentration was around...

02 May 2024 3,223 2 View

Itc experiments procedure?

I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...

28 April 2024 2,113 3 View