How to separate and quantify extra cellular polymeric substance (EPS) from co-culture involving bacteria and a filamentous fungi?

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James J. Smith

See publications. Generally centrifugation to pellet cells/conidia/hyphae, precipitation of supernatant in isopropanol at ca. 4oC, followed by dialysis at MWC ca. 5000 against distilled water .

Article Extracellular Polysaccharides from Desulfovibrio Desulfurica...

Article A sensitive chromatographic method for the detection of pyru...

Paranthaman Ramakrishnan

Dear Sir,

i think tis aricle will help

Kulandaivelu Velmourougane

Thanks Dr.Smith and Dr.Paranthaman for your inputs, the papers really helped me to understand the procedure. with regards