I am interested in quantifying quorum sensing in microbial biofilms. Is there any simple procedure to do so without using reporter bacteria
Biofilm formation among clinical isolates of Acinetobacter spp. was assessed and the production of signal molecules were detected with Chromobacterium violaceum CV026 biosensor system. Characterisation of autoinducers was carried out by mass spectrometric analysis. We have also reported the identification of an autoinducer synthase gene, abaΙ among the isolates that produce quorum sensing signal molecules and have reported that the mutation in the abaI gene influences their biofilm forming capabilities. Using a microtitre-plate assay it was shown that 60% of the 50 Acinetobacter spp. isolates significantly formed biofilms. Further detection with the biosensor strain showed that some of these isolates produced long chain signal molecules. Mass spectrometric analysis revealed that five of these isolates produced N-decanoyl homoserine lactone and two isolates produced acyl-homoserine lactone with a chain length equal to C12. The abaΙ gene was identified and a tetracycline mutant of the abaΙ gene was created and the inhibition in biofilm formation in the mutant was shown.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398020/
Dear Dr.
Thanks for your interest. But, i am looking for protocol to quantify quorum sensing in bacteria without using Chromobacterium violaceum.
with regards
You can use HPLC methodology to detect and quantify AHLs quorum sensing molecules in your samples.
https://www.ncbi.nlm.nih.gov/pubmed/24695235
Thanks Andrea for your interest. I need procedure to detect AHL without using Chromobacterium violaceum
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