Preparation of mM concentrations of amino acids
If you have only 1,0 mg of your amino acid you can make up ((1/molecular mass of amino acid) x 100) millilitres of a 10mM solution.
For example: 1mg glycine in 1.333 ml water or 1mg tyrosine in 0.552 ml water.
This is true for all the amino acids, but if you want to make up mixtures it will be a bit more complicated
Dear Prof.
Thanks for your answer
Simply use the following online calculator -
http://www.molbiol.ru/eng/scripts/01_04.html
Enjoy
Thank you Sir, this site is very useful
millimole= wt(mg)/Mr (where, Mr= Molecular mass of amino acid)
Conc. in millimoles dm^-3 = (wt(mg)/(Mr*vol in ml))*1000
In your case Conc. in millimoles dm^-3 = 10, Hence,
10= (wt(mg)/(Mr*vol in ml))*1000 --> vol. in ml =( wt(mg)/(Mr*10))*1000 = wt(mg)*100/Mr . So add wt*100/Mr ml of solvent to the amino acid.
Thanks you Sir for your help
you are welcome sir.
We have done whole transcriptome sequencing of bacteria and fungi. Is there any simple tutorial to submit these NGS data to NCBI.
17 June 2017 964 5 View
I have to do SEM analysis of root samples colonized by a selected bacterium. After removal of roots from the pots, how long I can store the samples in 2.5% Glutaraldehyde solution. Is there any...
08 December 2016 5,435 6 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View
I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...
25 February 2021 2,225 5 View
The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...
23 February 2021 2,049 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?
23 February 2021 3,053 7 View
I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....
22 February 2021 6,560 3 View