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Generally the rhizodeposition and its effects on soil-plant-microbial functions are quantified through isotope labeling. Is there any technique to quantify rhizodeposits without isotope labeling
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I have to study the tritrophic interactions between leafhopper + cotton plant + insecticides and its effects on trancriptome of leafhopper and cotton plants under controlled condition. How to...
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We have done whole transcriptome sequencing of bacteria and fungi. Is there any simple tutorial to submit these NGS data to NCBI.
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I have to do SEM analysis of root samples colonized by a selected bacterium. After removal of roots from the pots, how long I can store the samples in 2.5% Glutaraldehyde solution. Is there any...
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I am working on bacterial-fungal biofilm. I am testing the biofilm formation through crystal violet assay using microtitre plate. My doubt is, i am getting good biofilm formation between...
04 October 2016 392 5 View
In my biofilm study, i have extracted EPS and IPS. Different protocols are available on their storage (ultra pure water, buffers, distilled water, solvents etc.). Can anyone provide me simple...
15 July 2016 1,217 6 View
I have extracted EPS from biofilm study. However, the colour of the EPS is too dark and seems to have lots of debris. Is there any simple protocol to further purify them without affecting the EPS...
15 July 2016 9,201 3 View
I am working on bacterial-fungal biofilms. I have seen few papers on biofilm and EPS production by a few fungi. Is there any report on EPS production by Trichoderma sp.?
15 July 2016 1,342 6 View
I am working on bacterial-fungal biofilm. Is there any establised protocol to separate and quantify EPS from the co-culture.
03 July 2016 4,409 3 View
I have to do transcriptomic analysis of biofilm involving bacteria + filamentous fungi. There are procedures for transcriptomic analysis of biofilm involving bacteria-bacteria or bacteria-yeast...
31 March 2016 6,482 2 View
How to prepare Rhodamine B and Alcian Blue dyes for biofilm studies? What should be the solvents, concentration and storage procedure for the dyes?
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Which is the best solution to prepare amino acid stock solution? Sodium hydroxide/KOH/Distilled water?. After preparation, which will be the best condition to store amino acid solution and how...
09 January 2016 8,026 23 View
Preparation of mM concentrations of amino acids
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Is there any procedure available to study co-aggregation between bacteria and filamentous fungi during biofilm formation?
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Glyphosate degradation.
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