How to store the extracted extracellular and intracellular polysaccharides?

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Arvind Singh

Please have a look at these links and PDF attachment.

http://pubs.sciepub.com/jfnr/4/7/2/

http://jb.asm.org/content/191/23/7315.full

https://www.researchgate.net/publication/41213860_Optimising_Extraction_of_Extracellular_Polymeric_Substances_EPS_from_Benthic_Diatoms_Comparison_of_Efficiency_of_Six_EPS_Extraction_Methods

https://www.researchgate.net/publication/260113274_Extraction_and_measurement_of_extracellular_polymeric_substances_EPS_from_soil_biofilms_The_soil-microbial_interface

Article Optimising extraction of extracellular polymeric substances ...

Article Measuring the soil-microbial interface: Extraction of extrac...

Kulandaivelu Velmourougane

Thanks Arvind.

with regards

Marc Redmile-Gordon

Thanks Arvind,

I would add that it depends on your extractant. Kulandaivelu, this advice may come late but if using the dilute PBS as in Redmile-Gordon et al. (2014; 'extraction and measurement of EPS...' ref Arvind's post) then analyse within 24 hours. It is probably more convenient to freeze the extracts before analysis days/weeks/months later - If you have liquid nitrogen and -80 storage use this - if you don't then just place in -20 immediately after extraction. The sooner and faster these are frozen the better. Be sure to shake after defrosting.

Hope this helps,

marc

Dr. Marc, thanks for your clear suggestion. Presently, i have stored my samples in -20. One more clarification, my samples are very little in quantity (may be in micrograms), can i dissolve them in ultrapure water and store in -20?.

Thanks once again. with regards.

Velu

Difficult to say. I would just store mine in extraction buffer to be safe (as extracted) and avoid any dilution unless this was required immediately before analysis.

Thanks Dr.Marc