What is characteristic peak(s) in FTIR spectrum of crude plant extract?

Hello, I synthesized chitosan nanoparticles loaded with a plant extract. For confirming presence of the extract in the nanoparticles I did a FTIR analysis. Now my question is: What is...

31 December 2019 722 2 View

How to run a DNA-ligand docking in Molegro virtual docker?

Dear all, I would like to perform a DNA-Ligand docking in molegro virtual docker software. However, when the DNA imported in software it recognized as a big ligand instead of receptor. Is there...

31 December 2018 4,006 0 View

Which copper precursor if preferred for cu-nanoparticles synthesis in a green synthesis method?

Hi every one, I would like to synthesis of Cu Nanoparticles using plant extract, but in previous works different precursors have been used ( chloride and sulfate salts) , based on your experience...

09 October 2018 4,879 3 View

Which software is better for plotting of molecular dynamics stimulation results?

Dear researchers I am searching for a flexible and user friend software for plotting RMSD and RMSF curves except EXCEL. Could you suggest me a better software for my purpose. Thanks in advance for...

06 July 2018 6,818 6 View

How to interpret my HNMR spectrum? Is there any online software available to interpret the spectrum?

Hi, I have a HNMR spectrum of a sample (polymer+peptide) can anyone help me how can I interpret my HNMR spectrum

06 July 2018 3,116 0 View

Can anyone suggest me a journal in computational biology with rapid review processing?

Hi, I am looking for a journal in computational biology with rapid reviewing. Can anyone help me for this?

05 June 2018 1,117 2 View

Which in vitro analysis are available for evaluation of a potential vaccine efficacy?

Hi every one, I am working on a potential peptide based vaccine. In your opinion which in vitro analysis are available for evaluation of the vaccine efficacy. It should be mentioned that...

05 June 2018 206 6 View

How to fix this error in weka "train and test set are not compatible?

Hi every one I tried to perform a data classification in WEKA through SVM algorithm. There was no problem for train set and cross validation, but when I tried "supplied test set" this error was...

04 May 2018 942 27 View

Which HLA allele is dominant in world?

Dear All, I would like to evaluation efficacy of an antigenic peptide as a universal vaccine. For this, I want to performing a molecular docking between the peptide and Human HLA but due to high...

04 May 2018 1,895 1 View

Can anyone tell me how can I extract some number in text?

Hi, I have a text that include some number in latest line and I would like to extract this numbers to a separate file. Can anyone help me in this regard. A sample file of mu results is attached....

04 May 2018 1,800 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View