How to score with BIRADS a lesion which apperas benign at imaging but which is actually malignant?

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Publisher choice for a philosophy of language book proposal?

If you had to publish a book in philosophy of language-in-cognitive-sciences that is definitely not "continental" but also not strictly analytic, and discusses empirical (psychological,...

04 April 2024 4,436 2 View

How can we improve 260/230 ratio after DNA extraction from macroalgae?

Hi, We have extracted DNA from macroalgae samples with different kits and a CTAB protocol, but in every trial we obtained a low 260/230 ratio (the best has been 0.70). Does anyone know how to...

18 April 2023 1,379 0 View

Is 6xHis tag compatible with therapeutic (GMP) protein production?

Hello! We are producing a recombinant protein (from mammal cells) and we need a 6xHis tag for the purification. This protein is aim to be used in preclinical experiments in the short-medium term,...

11 January 2023 582 2 View

Sole cropping is still the dominant cropping system?

Despite the numbers of paper and researches showing the benefits of alternative cropping systems (intercropping, relay intercropping, mixed cropping), "sole cropping" with the due crop rotations...

10 November 2022 8,384 8 View

Has enthalpy of crystallisation/fusion of pure water been measured with DSC?

Hi all, I am looking for references about measuring crystallisation and metling enthalpy of pure water with differential scanning calorimetry (DSC). Although this seems to be quite a...

20 September 2022 3,191 5 View

Is it correct to express 1H NMR results as wt% ?

We have externalized some composition analysis of a thin silk polymer membrane that we produce in the lab. To solubilize the polymer we use a solution based on formic acid; Since formic acid is a...

17 August 2022 8,762 0 View

How to improve visualisation of oleosome membrane proteins with confocal microscopy?

Dear all, I am using confocal microscopy to visualise membrane proteins of oleosomes (in any case proteins surrounding the oleosome) extracted from rapeseed. The oleosomes have been stained with...

30 May 2022 9,203 4 View

How can I combine trees (.t files) from Mr.Bayes analyses using LogCombiner?

Dear all, I need to combine .t files from a MrBayes analysis. Using mcmcp, sumt, and sump commands is not an option, since due to CIPRES analytical limitations I need to combine files from 2...

21 April 2022 8,027 2 View

Is it possible to flip aminoacid sequences?

Goodmorning, I have a biomolecular dilemma that i can't answer. We would like to produce a fusion construct that is composed by two proteins in tail-to-tail conformation, otherwise one of the two...

05 January 2022 1,867 8 View

Formazan crystals dissolving during MTT assay?

Hi everyone, I'm having some trouble with classical MTT assay. Me and my group have been doing MTT assay for a few weeks now, then suddenly it started giving us problem. I'm working in 24-well...

28 March 2021 2,308 4 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

How to find projects for a team of professional developers in AI?

We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...

30 July 2024 8,377 0 View

Why do some of my Raman spectra show no signal at lower Raman shifts?

I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...

30 July 2024 8,771 3 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

What is single absorption ESR spectroscopy ?

In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?

25 July 2024 2,325 1 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

What is the physical meaning of the magnetic scalar potential?

In cases where the rotational of the magnetic field H is zero, we can define this field as the gradient of a scalar function defined as the magnetic scalar potential (similar to the electric...

21 July 2024 9,633 4 View

In Chip-seq, what will be the impact of adding proteinase K before removing the magnetic beads?

I was following this chip-seq protocol https://www.cellsignal.com/products/chip-kits-reagents/plus-sonication-chromatin-ip-kit/56383 what will be the consequence if on the following state "VI....

20 July 2024 5,612 0 View