Could expired BSA a problem for tissue culture?

Hi, I want to ask if for you using expired BSA powder (expired in 2014 but always in the fridge and protected from light) could be a problem for my bovine primary follicles culture in situ (so...

01 December 2020 8,650 2 View

Neyman - Scott Cluster Process: are these nonsensical parameter values ?

I am trying to fit a cluster model in spatstat in R using the locations of archaeological sites. The resulting parameters values are: trend: 2.514429e-06 kappa: 1.606992e-08 scale: 481.5851 mu:...

18 November 2020 2,993 3 View

Flow cytometry from harvested non-uniform tissue?

Hi everyone! I want to identify by flow cytometry the immune cells found in the brain with a localized injury. My hypotesis is that the injury will attract more/different immune cells respect to...

27 October 2020 748 2 View

What do you use to avoid insect collection infestation?

At the museum we are currently dismissing Paradichlorobenzene and Naphtalene. we are looking for other effective chemical or similar.

21 October 2020 9,553 11 View

Issue with plasmid cloning?

22 August 2020 1,687 15 View

Is there any commercial or open source DIC (2D or 3D) software which is capable of working on field photographs or satellite images?

Is there any commercial or open-source DIC (2D or 3D) software which is capable of working on field photographs of terrain or satellite images for deformation and strain analysis in geophysical...

11 August 2020 4,216 3 View

How to perform in silico peptide cyclization reactions by ChemAxon Reactor?

24 April 2020 9,398 1 View

Does the EGFP epression in a BAC-EGFP mouse correlate with the amount of promoter gene expression?

Hi, I am analyzing immunostainings of 5-HT2AR-EGFP transgenic mice (Htr2a Gene and 5-HT2A Receptor Expression in the Cerebral Co... ) and I have a doubt regarding the conclusion I can draw about...

21 July 2019 209 1 View

Is it possible to "paste" two functions in COMSOL?

Hi everyone, here is the description of the problem. I have a rectangle with some circular holes in it, the inner part of the domain (denoted with D) is defined by the difference between the...

07 July 2019 1,635 3 View

How to avoid for order effects in an experiment with 20 participants and one task with 12 trials?

Hey, we are studying the relationship between trait anxiety and spatial navigation. We have 20 participants and one navigation task with 12 trials. How to avoid for order effects among the trials?...

07 April 2019 9,479 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View