Hi there,

First you have to put the samples into liquid (sterile water or directly into rich medium)and then start culture into liquid rich medium (YEPD). Once it has grown you may isolate clones on YEPD plate by streaking. From one isolated clone, you may check actual strain phenotype towards auxotrophy markers an antibiotics and towards mutation (is it conditional mutant and/or is the mutation linked to a phenotype?). And once this is done and if it fits with the genotype given by your Chinese collaborators, I suggest you save the clone by first making a patch on plate and grow it and then transfer a maximum of cells into a screw cap tube containing YEPD + 15% glycerol and flashfreeze the sample in liquid nitrogen and then store it at - 80°C.

Thank you Dominique Liger.

my first mutant- K667 grows on medium: SD/-Leu/-Trp, another mutant  9.3 grows on  medium: SD/-His.

So i have prepared both broth and agar plates of these two SD medium and now i am thinking to put these Lyophilized yeast Cultures in respective SD broth and grow them at 28 C for 2-5 days. Once it becomes turbid and grows well i will put the 5ul of culture on respective SD agar plates and incubate them for 2-3 days to form colonies .

Is it correct? If I am wrong please correct me.

Hi,

strains are usually sent between labs spotted on sterile 3MM paper (small aquare ~ 1 cm2). In these condition they are quite stable and don't need to be stored a -20C. To retrieve cells I put the paper directly on a rich plate (YEPD) and incubate it at 25-30C (depends on mutations it carries) for few days, then you can (and should) check the phenotype as suggested by Dominique.

Harshraj,

I suggest that you to revive the yeast on rich medium (synthetic complete or YEPD) first, as advised by Dominique. Thereafter, if there are plasmids in your yeast strains that need to be maintained by continuous selection, restreak on to the appropriate synthetic dropout media. 

I've found that yeast strains can have a hard time forming colonies when they are revived directly on synthetic dropout medium.