9 Questions 22 Answers 0 Followers
Questions related from Harshraj Subhash Shinde
I did crisper cas9 experiment in plants, after creating the transgenic plants i analyzed the sequence for digestion and i saw digestion of single base at downstream of PAM site (5 base pair after...
21 September 2017 7,344 5 View
Doing 5' and 3' RACE with kit is bit expensive . can anyone share protocol of 5' and 3' RACE PCR without Kit or some literature regarding. I am trying to amplify unknown 3' and 5' region of my...
09 June 2016 974 4 View
I want to know weather is it possible to perform the PCR using oligo dt primer and Gene specific primer using RNA as a template? if possible then how to calculate the annealing temperature to set...
06 May 2016 3,267 3 View
After treating my gene and vector with RE. I tried to ligate them using DNA ligation kit (Takara. Cat. #: 6023). I am getting several colonies in plate but none of them is positive in colony PCR....
31 March 2016 9,879 10 View
To study the calcium and sodium transport function of one transporter gene I want to study them in k667 yeast mutant strain. So can anyone guide me me how and where to buy this strain? Thank you...
22 March 2016 5,186 4 View
I am trying to isolate a gene from rice whose size is 1.4 Kb. I have synthesized cDNA. Then In PCR of C-DNA I am getting amplification in House keeping gene (actin) but in case of my gene of...
27 January 2016 9,917 16 View
What is distribution of average sequence quality scores in RNA seq data analysis?
23 October 2015 1,027 2 View
Does any one knows how do read the dots of scatter plot while studying the gene expression level? Please guide by checking the attached file as a reference. Thank you.
23 October 2015 1,763 3 View
I am Searching collaborator for RNA seq. data analysis. As I am trying to learn about statistical analysis of RNA seq. data and finding it way difficult. If any one help me out in this can share...
16 October 2015 7,280 10 View
