16 Questions 37 Answers 0 Followers
Questions related from Harshraj Subhash Shinde
Hello all, I want to perform proteomic analysis in order to see the change in expression of cytokines for a particular trait. In order to do so I will need a internal standard for calibration....
15 May 2020 5,983 0 View
I have one more doubt about crisper cas9 experiment, I generated few transgenic plants for crisper cas9 mediated mutation, When I analyzed sequence for editing/mutation by sequencing I found that...
23 September 2017 6,607 10 View
I did crisper cas9 experiment in plants, after creating the transgenic plants i analyzed the sequence for digestion and i saw digestion of single base at downstream of PAM site (5 base pair after...
21 September 2017 7,459 5 View
I want to give salt treatment to my plants. I have grown them in soil in greenhouse (weight of soil in pot is 1.2 kg). I want to give 200mM NaCl stress treatment to these plant and then at...
01 August 2016 4,934 6 View
I am growing pearl millet in my lab. They are growing well both in hydroponics and soil. I want to give salinity stress to these plants by feeding them with NaCl. Then i want to isolate mRNA for...
16 July 2016 3,390 6 View
Doing 5' and 3' RACE with kit is bit expensive . can anyone share protocol of 5' and 3' RACE PCR without Kit or some literature regarding. I am trying to amplify unknown 3' and 5' region of my...
09 June 2016 1,026 4 View
I want to study the function of my gene in yeast i have transformed yeast with pyes2 vector containing my gene. I want to study the growth of transformed yeast under salinity stress so i am...
25 May 2016 9,870 15 View
I want to know weather is it possible to perform the PCR using oligo dt primer and Gene specific primer using RNA as a template? if possible then how to calculate the annealing temperature to set...
06 May 2016 3,336 3 View
I got the Lyophilized yeast Cultures of two yeast mutant strains send by another lab of china. Immediately after receiving the cultures I stored them at -20 But I don't know how to revive...
05 April 2016 5,083 4 View
After treating my gene and vector with RE. I tried to ligate them using DNA ligation kit (Takara. Cat. #: 6023). I am getting several colonies in plate but none of them is positive in colony PCR....
31 March 2016 9,963 10 View
To study the calcium and sodium transport function of one transporter gene I want to study them in k667 yeast mutant strain. So can anyone guide me me how and where to buy this strain? Thank you...
22 March 2016 5,240 4 View
I have isolated salinity related gene from halophyte and I have cloned them in pAUR123 (protein expression vector). Now I want to introduce them into yeast (Saccharomyces cerevisiae) to study the...
11 March 2016 3,655 8 View
I am trying to isolate a gene from rice whose size is 1.4 Kb. I have synthesized cDNA. Then In PCR of C-DNA I am getting amplification in House keeping gene (actin) but in case of my gene of...
27 January 2016 9,989 16 View
What is distribution of average sequence quality scores in RNA seq data analysis?
23 October 2015 1,083 2 View
Does any one knows how do read the dots of scatter plot while studying the gene expression level? Please guide by checking the attached file as a reference. Thank you.
23 October 2015 1,834 3 View
I am Searching collaborator for RNA seq. data analysis. As I am trying to learn about statistical analysis of RNA seq. data and finding it way difficult. If any one help me out in this can share...
16 October 2015 7,335 10 View
