How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How to define least effective concentration (LEC) and maximum safe concentration (MSC) of drugs?

In drug delivery, for efficacy, the drug concentration needs to be within LEC and MSC. How can we define that concentration?

25 November 2020 6,478 3 View

Perception on business failure prediction models using the annual report?

27 July 2020 1,141 2 View

Any suggestions for tools to analysis, plotting and tabulating genomic data of bacteria genomes?

Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance

27 July 2020 9,826 5 View

Dissertation Research: Business failure prediction. Could you help me with a short survey please?

I'm conducting a research study titled 'Perception on the business failure prediction using the annual report' to receive the degree of M.Sc. Accounting and Finance by Griffith College in Dublin,...

21 July 2020 8,761 3 View

Why does a small peptide precipitates in contact with albumin?

06 January 2020 8,673 5 View

Plotting my ordinal regressions results: which is the best graphic? Is it possible to use SPSS or Excel to plot this results?

I have many ordinal regressions with observed behavioural categories as VD (ordinal categories of 4 levels) and emotional state as VI (2 ordinal categories of 2 and 3 levels). I am using...

20 October 2018 5,243 3 View

What are the reasons for cell viability of more than 100% for DMSO control vehicle in MTT assay?

I performed MTT assay for assessing the cytotoxicity of curcumin (0-50uM) on LPS-stimulated THP-1 macrophages, and used DMSO as control vehicle. While calculating the cell viability% for DMSO, I...

26 June 2018 8,010 6 View

No title

As a control in my MAPK western blots I routinely use the anti-Fus3 antibody from Santa Cruz (SC-6772; YN-19SC), but recently learned that it has been discontinued from the catalogue. The same is...

07 February 2017 2,807 3 View

How can I retrieve lat and lon values of a Geotiff image?

Hi everybody, I try to extract values (lat and lon) of a Geotiff image that contains chlorophyll-a values, I need this information for compare with a MODIS values in MATLAB or another sofware.

08 September 2016 8,972 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View