Hello everyone!
I work in a project where I need to label lipids at the membrane, more specifically gangliosides, and now I would like to check the localization of these same lipids in relation to an intracellular protein. This means that I need to permeabilize the cell, but from my understanding permeabilization will remove lipids from the membrane, but I am not really sure about how big the impact on the membrane is.
Do you have any experience in this and is there a more appropriate method of fixation to try and maintain the membrane as intact as possible?
Thank you so much!