How can I prevent Ngn2 neuron-astrocyte co-cultures from peel off the coverslip?

Hello everyone, I've been co-culturing hiPSC-derived Ngn2 neurons with human fetal astrocytes, and at the time of grabbing the coverslip from the plate (24 wp) the cells peel off the coverslip. I...

03 September 2020 2,947 1 View

Any suitable DARPP-32(Thr34) antibody?

Does anyone know about any supplier for DARPP-32(Thr34) antibodies that work well in immunofluorescence? Specifically to prove in mouse and rat tissue.

01 February 2020 4,049 1 View

Anybody has access to this reference?

Hi everyone! I am working nowadays with bird cervical vertebrae and I haven trying to find this reference for a while, but it has been imposible to me to do it. As it is an old research is quite...

21 January 2020 1,774 2 View

Does anyone knows why output parameter is not working in this PyQgis script?

The problem must be the definition of the output string, because I tried the same code but writing a route directly on the parameters list and it worked. This is the code I wrote: import...

07 August 2019 6,108 4 View

Problem confirming discriminant validity (SmartPLS). Could you help me?

14 September 2016 4,923 9 View

Does UV-crosslink also work between proteins? And how is it possible to "reverse" it?

UV-crosslink is widely used to bind together proteins and RNA for co-isolation. Does it works also to connect proteins with other proteins? Is it possible to "reverse" such crosslink? For example,...

27 June 2016 7,546 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

31 December 2015 404 3 View

Why you have gone EC3 page "Emerging Sources Citation Index"?

10 November 2015 5,928 1 View

How to interpret vs. test in MCA analysis run by FactoMineR in R commander?

I did a MCA analysis using FactoMineR. I know how to interpret cos2, contributions and coordinates, but I don't know how values of v.test should be interpreted. Thank you

09 October 2015 8,383 3 View

Can anyone recommend a software that allows us to triangulate a raster image mapping, then add vector information for the triangles?

We want to restore an image using additional vector constraints.

25 February 2013 2,013 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View