I am trying to achieve a knock-out cells of these multiple myeloma cell lines. Then I want to realize a single cell culture of KO cells to obtain cell clones. Does anyone know if it is possible and what is the strategy to follow?
Thanks
I am attempting to ligate an oligonucleotide with a gene that has a double-stranded DNA sequence. Approximately 10-20% of the DNA forms gene dimers instead of ligating with the oligonucleotide....
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I need help identifying this ammonite species. It was found in the Oxfordian Ammonitico Rosso facies from the Balearic Islands, Spain.
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Dears, I developed a travel cost model comparing data which was collected before and after an ecological restoration. The aim is to determine if the restoration affected the recreational value of...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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