How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Is BRSV ("bovine respiratory syncytial virus") less susceptible to RNA degradation than SARS-CoV-2 when thawing a sample?

I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?

09 January 2021 4,168 7 View

Can you centrifuge and re-suspend a cDNA sample?

I am woking with very low concentrations of RNA in an attempt to run a qPCR analysis. Given this, I am going to attempt to synthesize cDNA without diluting my sample, and in two different,...

12 December 2020 3,247 3 View

Is the "Releve" method still standard protocol for plant community sampling and percent cover estimation?

I am not a botanist but need to estimate the average percent vegetation cover of my study area. I have researched this and found articles that use the "releve" method for determining plot size...

22 September 2020 5,676 6 View

How to accelerate Langevin dynamics simulations for bead-spring polymer chain models?

I'm running simulations for a bead-spring polymer networks using LAMMPS. I need my simulations to reach biologically relevant timescales of at least a few milliseconds. However each simulation...

14 September 2020 2,954 1 View

Convex Optimization for Deep Learning?

Is there such a thing as convex optimization for large deep learning networks? Is there a way to guarantee convergence? Has anyone looked into this?

06 August 2020 5,384 4 View

What happens in post-secondary education when instructors don't use traditional grades?

I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...

09 June 2020 1,519 6 View

What is the role of journals' reviewers? Should they be absolute judges?

- Ask questions where they are not sure/convinced about a statement/conclusion VS reject a statement/conclusion where they 'think' it is not correct? - Provide suggestions to improve a...

21 May 2020 2,624 7 View

Looking for immunologist or virologist to help with literature-based study for COVID treatment. Can you help?

On the side, I doing some research (literature-based) on a particular treatment that may help for COVID-19 symptoms but I need someone with a stronger medical knowledge base with expertise in...

29 April 2020 8,098 11 View

Female Soccer - Small sided games - Does training intensity reduce when elite players train with non elite ? or senior players with lower ages groups?

Hi , Im wondering does anyone know of any papers detailing the effects to training intensity of small sided games when the group is of mixed performance levels i.e( professional& semi...

18 December 2019 6,495 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View