How to remove fluorescent dye from proteins?

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The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What are the best paid maths journals?

Do they accept everything or they still pass them on a sieve?

12 January 2021 3,515 15 View

Why is Academia.edu so bad?

Is it even worth it using that site? Views are rare, the site malfunctions, is slow. Besides, their artificial "intelligence" algorithms get me wrong all the time, suggesting me lots of papers...

09 December 2020 7,296 6 View

Large Sample Size and Differences in Chi-Square Statistic?

Hi everyone, I am running a SEM and currently have a chi-square statistic of 5.188, with a sample size of 1765. I've read that the cutoff for the chi-square statistic should 3 or less. However,...

26 November 2020 3,109 4 View

Does anyone recommend any publication on the Japanese concept of "mabusabi" - specially refering to Shinohara Motoaki's works?

I am looking for materials that study Shinohara's concept of mabusabi and its demonstration in literature and visual arts.

10 November 2020 6,580 1 View

Find acoustic leaky modes with Comsol?

Hi, I need to estimate the overlap between optical modes and acoustic modes of a optically guiding and acoustically antiguiding waveguide (silica core suspended in air). I setup a Comsol model...

05 November 2020 6,507 2 View

Recent bibliography on Jesuits' dictionaries and "artes"?

Hello, colleagues, Greetings from Brazil. I would like to know if you could recommend recently published material (open access, preferably) addressing Jesuits' dictionaries and "artes" from 16th,...

21 October 2020 1,046 1 View

Can anyone help me in doing whole mount immunostaining on mouse colon?

I am exactly looking forward to know the procedure to isolate the myenteric and submucosal layer from intestinal whole mount. I would like to see the degeneration of dopaminergic neurons on it?...

12 October 2020 6,069 2 View

Standard curve and drug concentration for release behavior of drug?

I am working on drug loaded nanoparticles and their release behavior using dialysis membrane tubing method via UV-Vis spectrophotometer (nanodrop) and standard curve. For example, if i loaded 10...

17 September 2020 7,126 4 View

Formal equation for finding the Width and Gap calculation for CPW fed patch antenna?

As a beginner in antenna field , i come to know the design equation for Microstrip patch antenna from Balanis.But are there some equations to design the CPW fed patch antenna i.e. how one...

07 June 2020 5,239 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View