Hi everybody
I am Bita Divsalar, a Ph.D. candidate in hematology. My research focuses on creating an mRNA-based platform for CAR-NK cell therapy.
During my experiments, I encountered some difficulties in the RNA-LNP-mediated transfection of NK cells. In separate tests, we attempted to deliver mRNA encoding GFP and mRNA encoding BCMA-CAR-2A-GFP to HEK293T and NK-92 cell lines using a specific lipid nanoparticle (LNP). Our results showed that GFP was successfully delivered and expressed in HEK293T and NK-92 cell lines after transfection with GFP encoding mRNA. However, when BCMA-CAR-2A-GFP encoding mRNA was transfected, there was no evidence of GFP expression in NK-92 cells, despite an effective expression in HEK293T cells.
In subsequent trials, we endeavored to deliver two distinct sequences of BCMA-CAR to NK cells. But, unfortunately, neither of them was efficacious.
Our results surprised me and need some advice about this scenario. Could you kindly guide me with your opinion on the process of BCMA expression in NK cells?
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