Hello everyone,
I want to combine two genes by Gibson Assembly method, so I design primers for genes with 15nt homology; however, these two fragments have the same terminator, and the forward and reverse primers (30nt) share 10nt complementary to each other. And my PCR got no band when I run the product on gel electrophoresis; I guess the reason for this could be primer dimer. So is it work if I reduce the primer concentration? Or does anyone have other suggestions?
Thank you very much!