11 Questions 7 Answers 0 Followers
Questions related from Van Phung
Hello everyone, I did the gene transformation to produce a pigment. And I did an HPLC test to verify the pigment was produced. Then I want to examine the gene expression level using RT-PCR. I...
26 November 2021 8,292 5 View
Hello everyone, I want to do a RT-qPCR of Yarrowia lipolytica, however, I cannot find the CDS of actin gene in NCBI, does anyone know where to find it? PS: I saw some paper use actin gene for...
18 November 2021 9,286 0 View
Hello everyone, I have done a gene transformation, but when I do PCR for verification of my gene, there were so many bands, even I have used high annealing temperature (65 degrees). Could it be...
12 October 2021 1,319 7 View
Hello everyone, I am doing the URA excision, but my yeast not grow, what are possible reasons? do you have any suggestion? Thank you
27 August 2021 5,846 2 View
Hello everyone, I am doing yeast transformation? I should grow the starting yeast in 5-FoA/YPD to select URA(-) strains. Why do I need to do this step? Is it because URA(+) strains habouring URA...
13 April 2021 3,329 3 View
Hello everyone, I want to combine two genes by Gibson Assembly method, so I design primers for genes with 15nt homology; however, these two fragments have the same terminator, and the forward and...
05 March 2021 7,056 3 View
Hello everyone, I am searching information about increasing yield of secondary metabolites that are heterogeneously produce in S. cerevisiae. First I transfer several genes into yeast, then I...
02 March 2021 6,127 1 View
Hello everyone, I did several colony PCR, the enzyme I used here was Taq. In my first colony PCR, I set annealing temperature as 65 degree (which was similar to my basic PCR using KAPA, the...
08 February 2021 2,241 2 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
15 January 2021 6,710 2 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
28 December 2020 4,110 3 View
Hello everyone, my RNA-seq (conducted by the company) includes 2 pairs of samples. The RNA-seq results showed the comparison between two samples of the same pair, but not the cross comparison...
08 May 2017 5,573 3 View
