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Hello everyone, I did the gene transformation to produce a pigment. And I did an HPLC test to verify the pigment was produced. Then I want to examine the gene expression level using RT-PCR. I...
26 November 2021 8,241 5 View
Hello everyone, I have done a gene transformation, but when I do PCR for verification of my gene, there were so many bands, even I have used high annealing temperature (65 degrees). Could it be...
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Hello everyone, I am doing the URA excision, but my yeast not grow, what are possible reasons? do you have any suggestion? Thank you
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Hello everyone, I am doing yeast transformation? I should grow the starting yeast in 5-FoA/YPD to select URA(-) strains. Why do I need to do this step? Is it because URA(+) strains habouring URA...
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Hello everyone, I want to combine two genes by Gibson Assembly method, so I design primers for genes with 15nt homology; however, these two fragments have the same terminator, and the forward and...
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Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
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Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
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