Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

Fixation of whole rats organs in buffered formalin?

Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman

28 October 2020 3,175 3 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

If you had to reference a publication which proves or at least rigorously argues that AI control problem is (un)solvable, what would you cite?

Links to such articles with some explanations would be great!

27 July 2020 7,035 6 View

Perception on business failure prediction models using the annual report?

27 July 2020 1,141 2 View

Dissertation Research: Business failure prediction. Could you help me with a short survey please?

I'm conducting a research study titled 'Perception on the business failure prediction using the annual report' to receive the degree of M.Sc. Accounting and Finance by Griffith College in Dublin,...

21 July 2020 8,761 3 View

What is the estimated projected price of electricity (or hydrogen) for bio-solar cells?

Hello, everyone! There are many reports about bio-based solar cells and their prospects in the scientific literature. All authors write that these devices will be low-cost. Does anyone know any...

13 July 2020 655 3 View

Whas is the ideal pathway for making an economic assesment for hidrologic ecosystem services?

17 April 2020 5,969 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View