What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

Do you consider that rose bengal chloramphenicol agar is suitable to isolate and count fungus?

Someone knows about a specific law or regulation which mentions the use of the rose bengal chloramphenicol agar is suitable to isolate and count fungi. Perhaps, someone has done a research about...

07 January 2021 5,325 5 View

Regression analysis on small but complete dataset (perfect representation of reality)?

I have a repeated measures design with about 16 cases and 5-6 points of measuring. Sometimes, 1-4 full cases or some points of measure are missing. (The measures are 20 numerical and categorical...

20 December 2020 9,004 7 View

Approval for MnFe2O4 for industrial wastewater treatment?

I am using MnFe2O4 NPs as adsorbent for CrVI for wastewater treatment following literatures as it is one of the best, but I have a challenge of releasing Mn+2 in the environment. A lot of...

19 November 2020 3,404 4 View

FISH probe synthesis with PCR?

Hello, I have some troubles with the synthesis of a DNA probe for FISH in paraffin tissues, we decide to synthesize it by PCR with labelled dUTP, but we haven't had any results, I tried chaging...

08 September 2020 183 3 View

HAP1 cell transfection with RNP complexes?

Hi, Does anyone have experience with transfecting HAP1 cells for genome editing with Cas9/gRNA complexes? Or are the HAP1 cells difficult to transfect? Thanks.

27 August 2020 6,028 1 View

Psychologically, How can you support a Covid-19 patient ?

After being affected with that viruse , most of the patients feel depressed and sad . How can we , psychologically, encourage them ?

26 August 2020 6,178 93 View

How does COVID-19 affect the Stakeholder Engagement of YOUR EU Energy OR Marine Projects?

Dear researchers, The COVID-19 pandemic has large societal and economic impacts, from which also different stakeholders involved in EU H2020 energy projects are affected. With this survey, we...

17 June 2020 4,542 4 View

MIKE 21 Illegal Storage Access?

Has anyone ever received the error 'Illegal storage access Abnormal completion' from MIKE? I am using the Data Extractor FM and at certain times this error comes up. Once I receive this error I...

24 March 2020 2,518 3 View

How to test an old cell culture?

We have a HepG2 culture and want to test it on possible mutation. Can we verify culture by PCR for example? And what genes can we use for this?

23 March 2020 3,175 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View