Flow cytometry - fluorescence intensities for individual cells?

Hi All, I am new to flow cytometry so please bear with me :) Is there a way to retrieve fluorescence intensities for individual cells from an .fsc file form a flow run? Basically, I am looking...

31 December 2018 3,770 3 View

T-cell receptors interacting specifically with acetylpeptides?

Hi, Is anyone aware of any publications, in which T-cells with T-cell receptors interacting specifically with acetylpeptides but not unmodified peptides, were reported? Thank you. Best, Martyna

05 June 2017 1,657 1 View

Cancer vaccines

Hi, How do cancer vaccines actually work? If I inject a peptide neoantigen into a mouse, what happens to it? Is it engulfed by the antigen presenting cells? if so, is it then presented on MHC...

05 June 2017 9,420 3 View

Peptide pulsing of dendritic cells

Hi, I was wondering, what are the controls one would use for pulsing peptides into naive in vitro derived dendritic cells? How can I verify that the pulsed peptide is actually being presented on...

05 June 2017 6,054 3 View

Why are some tumor associated antigens (TAAs) cancer-type specific?

For example, MAGE-1 is a TAA commonly associated with melanoma, and WT1 in renal cancer - what dictates this tissue/cancer type specificity?

04 May 2017 9,413 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View