Flow cytometry - fluorescence intensities for individual cells?

Hi All, I am new to flow cytometry so please bear with me :) Is there a way to retrieve fluorescence intensities for individual cells from an .fsc file form a flow run? Basically, I am looking...

31 December 2018 3,770 3 View

How to recover high quality gDNA following cell fixation and permeabilization for flow cytometry?

Hi, I want to sort cells based on expression of several intracellular proteins. To do so, I need to fix and permeabilize my cells. I was wondering if anyone has experience in this type of...

02 March 2018 5,363 0 View

Cancer vaccines

Hi, How do cancer vaccines actually work? If I inject a peptide neoantigen into a mouse, what happens to it? Is it engulfed by the antigen presenting cells? if so, is it then presented on MHC...

05 June 2017 9,420 3 View

Peptide pulsing of dendritic cells

Hi, I was wondering, what are the controls one would use for pulsing peptides into naive in vitro derived dendritic cells? How can I verify that the pulsed peptide is actually being presented on...

05 June 2017 6,054 3 View

Why are some tumor associated antigens (TAAs) cancer-type specific?

For example, MAGE-1 is a TAA commonly associated with melanoma, and WT1 in renal cancer - what dictates this tissue/cancer type specificity?

04 May 2017 9,413 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View