S1 PFGE for visualizing megaplasmids, what differs from a ususal PFGE (other than using S1 instead of res enzyme)?

The question is about S1 PFGE ; a pulsed field in which S1 is used to linearize plasmidic DNA so that the latter could migrate through pulsed elecrophoresis. i intend to run a usual PFGE, i wanted...

02 March 2018 1,037 2 View

I would like to know the impact factor of 'Journal of Chemical Biology' ??

09 October 2015 5,319 1 View

Why use mineral oil in RAPD experiments and which one?

It is indicated that RAPD reactions are made with a layer of mineral oil on the top of the mixture, iI would like to ask which oil to use and how and why it is used? I actualy did the experiment,...

09 October 2014 2,437 4 View

how could a badly designed primer pair affect a PCR (conventional and quantitative)?

04 May 2014 4,800 3 View

Which free journals are there in topics of molecular microbiology with impact factor between 1 and 2?

Our study investigated a real time PCR technique as a diagnostic tool, and for virulotyping (screnning of virulence genes) by real time PCR; can anyone suggest some journals (free of charges plz)...

01 February 2014 4,637 5 View

A question about your choice. Why wild animals?Are you searching just for financial support or you need cooperating laboratories to do the analyzis?

no details needed

01 January 1970 4,404 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View